2292 2295 2296 2044 2312 2301
Protein Expression
[edit]2014/9/5
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pT7A3E-yPolyA-Pol |
2292 |
15 ml, Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
s |
7.63/66.2 |
yes | |
| 2 |
pET30b-yPolyA-Pol |
2295 |
15 ml,
Nico21(DE3)plysN |
0.4mM IPTG RT O/N |
s |
7.63/66.2 |
yes | |
| 3 |
pET28b-Exo-VII |
2296 |
15 ml,
Nico21(DE3)plysN |
0.4mM IPTG RT O/N |
s |
9.94/52.9 |
no | |
| 4 |
pageRuler Plus Prestained protein ladder |
|||||||
| 5 |
pBad24-Tat-yFLP |
2044 |
15 ml,
TOP10plysN |
10mM Arabinose RT O/N |
s |
|||
| 6 |
15 ml,
Nico21(DE3)plysN |
10mM Arabinose RT O/N |
s |
|||||
| 7 |
pEB-iFLP |
2312 |
15 ml,
TOP10plysN |
10mM Arabinose RT O/N |
s |
|||
| 8 |
15 ml,
Nico21(DE3)plysN |
10mM Arabinose RT O/N |
s |
|
||||
| 9 |
15 ml, Tuner(DE3)plysN |
10mM Arabinose RT O/N |
s |
|
||||
| 10 |
pR-wtSM2-NucA |
2301 |
Ecoli K12 JM101 |
QHP |
concentrated |
|||
| 11 |
||||||||
| 12 |