2302 pSBGFP
Cloning of 2302 pSBGFP
Purpose: Cloning of2302 pSBGFP
Date: 2014-8----
Notebook:
Page:
Clone ID:2302
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2286 using
2302-SbGFP-Nhe1-F tacacgct agc gccaccATGGTGAGCAAGGGCGAG
2302-SbGFP-BamH1-R CGGTGGATCCTTCTGGAAGAAGAAATTATAAACTCCGCTTCCCTTGTACAGCT
(AGCTGTACAAGGGAAGCGGA GTT TAT AAT TTC TTC TTC CAG AAG GAT CCA CCG)
digest with BamH1 + Nhe1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use BamH1 + Nhe1 to find positive clone
primer design was wrong, there is no BamHI site in 2218
Cloning of 2302 pSBGFP
[edit]Purpose: Cloning of2302 pSBGFP==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2302
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2286 using
2302-SbGFP-Kpn1-F tacacGGTACC gccaccATGGTGAGCAAGGGCGAG
2302-SbGFP-Nhe1-R GGTAGCGCTAGCTTCTGGAAGAAGAAATTATAAACTCCGCTTCCCTTGTACAGCT
digest with Kpn1 + Nhe1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Kpn1 + Nhe1 to find positive clone
Transfection
