2319 pEB3

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Cloning of 2319 pEB3

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Purpose: Cloning of2319 pEB3==

Date: 2014-9-15---

Notebook:0

Page:

Clone ID:2319

Experimenters:  shanyi

Results

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CLONING

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PCR pBad promoter using pBad-Age1-F AACCAAACCGGTAACCCCGCTTAT

2319-pBadP-Nde1-R (CATACCCGTTTTTTTgggctagaaataattttgtttaactttaagaaggagatatacatatgtagca)

    tgctacatatgtatatctccttcttaaagttaaacaaaattatttctagcccAAAAAAACGGGTATG


digest with Nde1 + Age1


Colony Screening

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Date: 2014_9

Make miniprep, useNde1 + Age1 to find positive clone


Result intepretation/Summary

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Conclusion

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