2320 pFastBacDual-VSVG-Flp
Cloning of 2320 pFastBacDual-VSVG-Flp
[edit]Purpose: Cloning of2320 pFastBacDual-VSVG-Flp==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2320
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone Flpo from 1981 pCMV5-Tat-Flp-PDGFR to 2224 pFastbacDual-p10-VSVG-M using Nhe1 + Xba1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nhe1 + Xba1 to find positive clone
Cloning of 2320 pFastBacDual-VSVG-Flp
[edit]Purpose: Cloning of2320 pFastBacDual-VSVG-Flp==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2320
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone Flpo from 1981 pCMV5-Tat-Flp-PDGFR to 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8using Nhe1 + Xba1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use BamHI + Xba1 to find positive clone
Cloning of 2320 pFastBacDual-VSVG-Flp
[edit]Purpose: Cloning of2320 pFastBacDual-VSVG-Flp==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2320
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone Flpo from 1981 pCMV5-Tat-Flp-PDGFR to 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8usingBamH1 + Xba1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use BamHI + Xba1 to find positive clone
