406 1560 1074 1706 1905 2003 2033 2040 2080 20140512

Protein Expression

2014/5/12
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pET28b-Int	Int	406	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	8.82/42.1	?
2	pT7A3E-T4-Endo-IV	T4-Endo-IV	1560	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s+P	6.35/22.68
3	pET28b-IHFab	IHFab	1074	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	S	6.63/13.65
4	pT7A3-BirA	BirA	1706	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	7.76/35.3	YES
5	pT7A3E-TBSV-p19	TBSV-p19	1905	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.3/19.39
6	pT7A3-8H-TBSV-p19	8H-TBSV-p19	2003	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s
7	pLC6H-DrpSSB	DrpSSB	2033	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	4.71/35.76
8	ageRuler Plus Prestained protein ladder
9	pT7A3-6H-sso7d-wt-Taq	sso7d-wt-Taq	2040	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	S+P	6.24/104.1
10	pT7A3-6H-sso7d-wt-Taq	sso7d-wt-Taq	2040	15 ml, Tuner(DE3)plysN	0.3mM IPTG RT O/N	S	6.24/104.1	YES
11	pT7A30-wt-Taq-sso7d	wt-Taq-sso7d	2080	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	S+p	6.24/104.1
12	pT7A30-wt-Taq-sso7d	wt-Taq-sso7d	2080	15 ml, Tuner(DE3)plysN		S	6.24/104.1