1673 1710 2267 2324 2332 2343 2345 637
Protein Expression
[edit]2014/9/
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Induction |
SAMPLE |
pI/Mw |
express |
| 1 |
pET28b-NdeI |
NdeI |
1673 |
15 ml, Nico21(DE3)plysN |
0.4mM IPTG RT O/N |
s |
8.6/46.4 |
yes |
| 2 |
pET28b-NdeI |
NdeI |
1673 |
15 ml,
Tuner(DE3)plysN-ha-plac-hia5 |
0.4mM IPTG RT O/N |
s |
yes | |
| 3 |
pT7A3-NdeI |
NdeI |
1710 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
S |
yes | |
| 4 |
pT7A3-NdeI |
NdeI |
1710 |
15 ml,
Tuner(DE3)plysN-ha-plac-hia5 |
0.2mM IPTG RT O/N |
s |
YES | |
| 5 |
||||||||
| 6 |
pT7A3-Tth-PPase |
Tth-PPase |
2267 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
s |
5.6/20.9 |
yes |
| 7 |
pL6H-sso7d-wt-Taq |
sso7d-wt-Taq |
2324 |
15 ml,
Nico21(DE3)plysN |
42oC O/N |
s |
6.26/104.7 |
YES, 42oC O/N inducing is too long, cell lysed |
| 8 |
pT7A30-Cas9 |
Cas9 |
2332 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
s |
9.0/164 |
yes |
| 9 |
pET28b-T4-endo-VII |
T4-endo-VII |
2343 |
15 ml,
Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
S |
8.9/20.3 |
yes |
| 10 |
pCold-HCMVUL98 |
HCMVUL98 |
2345 |
15 ml,
15 ml, Nico21(DE3)plysN |
0.2mM IPTG RT O/N |
S |
6..03/67.1 |
no |
| pT7A3-NcoI |
637 |
15 ml,
Tuner(DE3)plysN-ha-plac-hia5 |
0.2mM IPTG RT O/N |
S |
5.6/31.5 |
no | ||
| 12 |
pT7A3-NcoI |
637 |
15 ml,
Top10plysN-ha-plac-hia5 |
S |
5.6/31.5 |
no |