2297 pEC2-NotI
Cloning of 2297 pEC2-NotI
[edit]Purpose: Cloning of2297 pEC2-NotI==
Date: 2014-7--2014/8/1--
Notebook:0
Page:
Clone ID:2297
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 567
Not1-Xba1-F atacactctagaTGCGTAGCGATACCAGCGTTG
Not1-HindIII-R (CAGCGTGGTCTGTTTGATGCA CACCACCACCACCACCACTGA AAGCTT) AAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG
digest with Xba1 + HindIII, clone to 2015 pEC2
Colony Screening
[edit]Date: 2014_8_1
Make miniprep, use Xba1 + HindIII to find positive clone
Cloning of 2297 pEC2-NotI
[edit]Purpose: Cloning of2297 pEC2-NotI==
Date: 2014-8
Notebook:0
Page:
Clone ID:2297
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 567
Not1-Xba1-F atacactctagaTGCGTAGCGATACCAGCGTTG
Not1-HindIII-R (CAGCGTGGTCTGTTTGATGCA CACCACCACCACCACCACTGA AAGCTT) AAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG
digest with Xba1 + HindIII, clone to 2015 pEC2
Colony Screening
[edit]Date: 2014_8_8
Make miniprep, use Xba1 + HindIII to find positive clone
there are two HindIII site in the 2015 vector, so cannot use pEC2 to do this clone, use 1454pEC instead.