2334 pCMV-Cas9-Nickase

From ELN
Revision as of 13:29, 3 October 2014 by Excellgen (talk | contribs) (Created page with "== '''Cloning of 2334 pCMV-Cas9-Nickase''' == '''Purpose:''' Cloning of2334 pCMV-Cas9-Nickase'''==''' '''Date: '''2014-9---- '''Notebook:0''' '''Page:''' '''Clone ID:23...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 2334 pCMV-Cas9-Nickase

[edit]

Purpose: Cloning of2334 pCMV-Cas9-Nickase==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2334

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from 2275 CAS9P using

2334-hCas9-D10A-Nhe1-F tagcacGCTAGCCGCCACCATG GAC AAG AAG TAC AGC ATC GGC CTG GcC ATC GGC AC
2334-Cas9-BamH1-R ATGGCGGGGGATCCGGCCAGATTG


digest with Nhe1 + BamH1, clone to 2275 CAS9P



Colony Screening

[edit]

Date: 2014_8

Make miniprep, use  Nco1 + BamH1 to find positive clone




Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2334_pCMV-Cas9-Nickase&oldid=13346"