2335 pET28b-Cas9-C

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Cloning of 2335 pET28b-Cas9-C

Purpose: Cloning of2335 pET28b-Cas9-C==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2335

Experimenters: shanyi

Results

CLONING

PCR from 2275 CAS9P

2335-Cas9-BamH1-F CAATCTGGCCGGATCCCCCGCCAT

2335-Cas9-Xho1-R (GACCCCAAGAAAAAGCGCAAAGTG CTCGAG tagca) tgctaCTCGAGCACTTTGCGCTTTTTCTTGGGGTC

digest with XhoI + BamH1, clone to V12

Colony Screening

Date: 2014_8

Make miniprep, use XhoI + BamH1 to find positive clone

Result intepretation/Summary

Conclusion

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Cloning