2323 pR-Enterokinase
Cloning of 2323 pR-Enterokinase-
[edit]Purpose: Cloning of2323 pR-Enterokinase-==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2323
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 1731 pT7A3-TurboEnterokinase
Frag 1. 2323-EK-Nde1-F C1 tagca catatg attgtcggaggaagtgactc+ 2323-EK-R1 C3 tgatgccatGtgcaggcctag
Frag 2 2323-EK-F1 C2 gcctgcaCatggcatcaaatc +Enteokinase-Xho1-R gtatcgCTCGAGCTAgTGATGATGATGATGATGTAGAAAACTTTGTATCCACTCTG
join two fragments by PCR 2323-EK-Nde1-F C1 tagca catatg attgtcggaggaagtgactc Enteokinase-Xho1-R gtatcgCTCGAGCTAgTGATGATGATGATGATGTAGAAAACTTTGTATCCACTCT
digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]