2350 pFastbac-GP64

From ELN
Revision as of 11:58, 29 October 2014 by Excellgen (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cloning of 2350 pFastbac-GP64

[edit]

Purpose: Cloning of2350 pFastbac-GP64==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2350

Experimenters:  shanyi

Results

[edit]

CLONING

[edit]

PCR from 2351 pEC2-GP64

Fragment 1

2350-GP64-Xho1-F tacgac ctcgag ATGGTAAGCGCTATTGTTTTATATG

2350-GP64-R1 (gaaaccgtaatagacaatattaa gtac) gtacttaatattgtctattacggtttc


Fragment 2

2350-GP64-Xho1-F tacgac ctcgag ATGGTAAGCGCTATTGTTTTATATG

2350-GP64-R2 (gaaaccgtaatagacaatattaa) ttaatattgtctattacggtttc


purify two fragments in one tube, heat to 95 oC, cool slowly to RT then digest with Xho1 (without Kpn1)

ligate to Xho1 + Kpn1 digested vector



Colony Screening

[edit]

Date: 2014_8

Make miniprep, use Xho1 + Kpn1 to find positive clone





Result intepretation/Summary

[edit]

Conclusion

[edit]
Retrieved from "https://eln.excellgen.com/index.php?title=2350_pFastbac-GP64&oldid=13487"