1916 pEC2-M-SssI

Cloning of 1916 pEC2-M-SssI-

Purpose: Cloning of1916 pEC2-M-SssI-==

Date: 2014-9----

Notebook:0

Page:

Clone ID:1916

Experimenters:  shanyi

Results

CLONING

gene synthesis fragment 1,Fragment 2

join two fragments by PCR

digest with Nde1 + Xho1

Colony Screening

Date: 2014_8

Make miniprep, use  Nde1 + Xho1to find positive clone

fix 1916-2 pEC2-M-SssI

pcr

jOIN two fragment by PCR

digest with Nde1 + XhoI

Colony Screening

Date: 2014_8

Make miniprep, use  Nde1 + Xho1 to find positive clone

use T7 term primer+T4-BGT-2-16 to do PCR

sequence 1916fix-1 not fixed,need to fix again

fix 1916-2 pEC2-M-SssI again

pcr

jOIN two fragment by PCR

digest with Nde1 + XhoI

36_pEC2-mNco1

Colony Screening

Date: 2014_8

Make miniprep, use  Nde1 + Xho1 to find positive clone

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