2371 pEC2-Sirt1

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Cloning of 2371 pEC2-Sirt1

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'Purpose:Cloning of2371 pEC2-Sirt1==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2371

Experimenters:  shanyi

Results

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CLONING

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PCR from 2370 pENTR223-Sirt1

2371-SIRT1-Nde1-F1 C6 attcaccatatgGCTAGCCACCATGGCGGCGGCAGCGGCGGCGGCAGCGATC

1 ul of 1 uM 2371-SIRT1-Nde1-F2 C7 GCAGCGGCGGCGGCAGCGATCGGCTACCGAGGTCCATATACTTTTGTTCAG

2371-SIRT1-Kpn1-R C8 tgtctaGGTACCGTTTAAACTTATGATTTGTCTGATGGATAG


DIGEST WITH Nde1 + Kpn1,CLONE TO 2015

Colony Screening

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Date: 2014_9

Make miniprep, use Nde1 + Kpn11to find positive clone




Result intepretation/Summary

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Conclusion

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