Baculovirus Cre FLP vlpQC cre QC 20141103
VLP from sf9 cells.
Original FLP: VLP in sf9 cell culture medium
Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration, then ultrafiltrated again, filtered on 10/10/2014
Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration , filtered on 10/08/2014
Final Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration ,then ultrafiltrated again, filtered on 10/10/2014
Reporter: 2013 for Flp, 2181 for Cre
Oct. 30 2014 Morning : split 293 cells
Oct. 30 2014 afternoon Transfect 293 cell (70--80%) using lipofectamin 2000
Oct.31 2014 10:00 am: 70%-80% cells turns red , cells are in good condition, add Flp and Cre to reporter cells
|
A1: 75ul original FLP |
A2: 50ul Final Purified FLP (filtered on 10/10/2014) |
A3: 75ul Purified cre (filtered on 10/8/2014) |
A4: 50 ul Final Purified FLP (filtered on 10/10/2014) |
A5: 1uM cre lot#: 20130826 |
A6: 2.5uM cre lot#: 20130826 | ||
| B1:
0ul Final Purified FLP (filtered on 10/24/2014) |
B2:
75ul Final Purified FLP (filtered on 10/242014) |
B3:
0ul Final Purified FLP (filtered on 10/29/2014) |
B4:
50ul Final Purified FLP (filtered on 10/10/2014) |
B5: 1uM cre lot#: 20131115 |
B6: 2.5uM cre lot#: 20131115 | ||
|
C1: 100ul Final Purified FLP (filtered on 10/24/2014) |
C2:
150ul Final Purified FLP (filtered on 10/24/2014) |
C3:
75ul Final Purified FLP (filtered on 10/29/2014) |
C4:
100ul Final Purified Cre (filtered on 10/29/2014) |
C5:
1uM cre lot#: 20131219 |
C6:
2.5uM cre lot#: 20131219 | ||
| D1:
200ul Final Purified FLP (filtered on 10/24/2014) |
D2:
250ul Final Purified FLP (filtered on 10/24/2014) |
D3:
150ul Final Purified FLP (filtered on 10/29/2014) |
D4:
150ul Final Purified FLP (filtered on 10/29/2014) |
D5: 1uM cre lot#: 56375 |
D6: 2.5uM cre lot#: 56375 |
Nov.3
