2383 pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H

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Revision as of 18:20, 20 November 2014 by Excellgen (talk | contribs) (Created page with "== '''Cloning of2383 pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H''' == '''Purpose:''' Cloning of2383 pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H'''==''' '''Date: '''2014-9---- '''Notebook:0''' ...")

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Cloning of2383 pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H

Purpose: Cloning of2383 pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H==

Date: 2014-9----

Notebook:0

Page:

Clone ID:2383

Experimenters: shanyi

Results

CLONING

subclone FLP from 2203 pCold-MBP-Tat-NLS-iFLP-6H to 2197 pT7A3-6XHis-Sumo Nde1 + Xho1

Colony Screening

Date: 2014_8

Make miniprep, use Nde1 + Xho1 to find positive clone,will get two bands

Result intepretation/Summary

Conclusion

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Cloning