2381 pT7A3-TRX-Tat-NLS-iFlp-6H
Cloning of2381 pT7A3-TRX-Tat-NLS-iFlp-6H
[edit]Purpose: Cloning of2381 pT7A3-TRX-Tat-NLS-iFlp-6H==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2381
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone FLP from 2203 pCold-MBP-Tat-NLS-iFLP-6H to 1833 pT7A3-TRX using Nde1 + Xho1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1 to find positive clone,will get two bands