Cre 751708 761708 flp QC 2014 11 19-21
11/17/2014: transfect 293 cells in 24 well plate with 2181 (for cre) and 2013(for flp)
11/18/2014: add recombinase
freeze-dried cre incubated at 37oC for 3 days and put in RT for two days
|
Flp negative control 2013 |
Flp positive control 75ul original Flp VLP |
2uM Flp from cell culture medium |
5uM Flp
from cell culture medium |
2uM Flp
from 293 cell |
5uM Flp
from 293 cell |
| CRE negative control
2181 |
CRE positive control
2.5uM CRE (lot#56-375) |
2.5uM 1st time freeze-dried cre (0%Trehalose,5%glycerol) |
2.5uM 1st time freeze-dried cre (5%Trehalose,5%glycerol) |
2.5uM 1st time freeze-dried cre (10%Trehalose,5%glycerol) |
2.5uM (15%Trehalose,5%glycerol) |
| 2.5uM cre control 4oC (0%Trehalose) |
2.5uM 2nd time freeze-dried cre (0%Trehalose) |
2.5uM 2nd time freeze-dried cre (5%Trehalose) |
2.5uM 2nd time freeze-dried cre (10%Trehalose) |
2.5uM 2nd time freeze-dried cre (15%Trehalose) |
CRE negative control
2181 |
| 1uM CRE
(lot#75-1708) |
2.5uM CRE
(lot#75-1708) |
5uM CRE
(lot#75-1708) |
1uM CRE
(lot#76-1708) |
2.5uM CRE
(lot#76-1708) |
5uM CRE
(lot#76-1708) |
11/19/2014: pictures under microscope 24HOURS CELL ARE IN GOOD CODITION
11/20/2014 48HOURS
|
Flp negative control 2013 |
Flp positive control 75ul original Flp VLP |
2uM Flp from cell culture medium CELL DIED |
5uM Flp
from cell culture medium CELL DIED |
2uM Flp
from 293 cell CELL DIED |
5uM Flp
from 293 cell CELL DIED |
| CRE negative control
2181 |
CRE positive control
2.5uM CRE (lot#56-375) |
2.5uM 1st time freeze-dried cre (0%Trehalose,5%glycerol) |
2.5uM 1st time freeze-dried cre (5%Trehalose,5%glycerol) |
2.5uM 1st time freeze-dried cre (10%Trehalose,5%glycerol) |
2.5uM (15%Trehalose,5%glycerol) |
| 2.5uM cre control 4oC (0%Trehalose) |
2.5uM 2nd time freeze-dried cre (0%Trehalose) |
2.5uM 2nd time freeze-dried cre (5%Trehalose) |
2.5uM 2nd time freeze-dried cre (10%Trehalose) |
2.5uM 2nd time freeze-dried cre (15%Trehalose) |
CRE negative control
2181 |
| 1uM CRE
(lot#75-1708) |
2.5uM CRE
(lot#75-1708) |
5uM CRE
(lot#75-1708) CELLS ARE NOT IN GOOD CONDITION |
1uM CRE
(lot#76-1708) |
2.5uM CRE
(lot#76-1708) |
5uM CRE
(lot#76-1708) CELLS ARE NOT IN GOOD CONDITION |
11/21/2014 72HOURS, most of the cells are not in good condition
