2382 pT7A3-6XHis-SMT3-(Tev)

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Cloning of2382 pT7A3-6XHis-SMT3-(Tev)

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Purpose: Cloning of2382 pT7A3-6XHis-SMT3-(Tev)==

Date: 2014-11----

Notebook:0

Page:

Clone ID:2382

Experimenters:  shanyi

Results

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CLONING

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CR from 2285 pBY011-SMT3

2197-SUMO-Nco1-F1 tcgac cc atg ggc agc agc cac cat cac cat cac cat tcggactcagaagtcaatc

2382-SMT3_TEV_Nde1-R (cacagagaacagattggtggt ggggatgacgatgacaaggaaaacctttacttccagggccatatg cat atg tagcac) gtgctacatatgcatatggccctggaagtaaaggttttccttgtcatcgtcatccccaccaccaatctgttctctgtg



Digest with Nde1-Nco1


Colony Screening

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Date: 2014_11

Make miniprep, use Nde1-Nco1 to find positive clone


Result intepretation/Summary

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Conclusion

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