2388 pET30b-Gst-PcrA
Cloning of2388 pET30b-Gst-PcrA
[edit]Purpose: Cloning of2388 pET30b-Gst-PcrA==
Date: 2014-12----
Notebook:0
Page:
Clone ID:2388
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from Geobacillus stearothermophilus NUB3621 genomic DNA
2388-Gst-PcrA-Nde1-F A1 tagcag catatga attttttgtc ggaaaaatta ctg
2388-Gst-PcrA-Xho1-R A2 cgtacactcgagcacttttgtaatcggagcaaac
Digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_12
Make miniprep, use Nde1 + Xho1to find positive clone
