2390 pT7A3E-Gst-MutL

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Cloning of2390 pT7A3E-Gst-MutL

Purpose: Cloning of2390 pT7A3E-Gst-MutL==

Date: 2014-12----

Notebook:0

Page:

Clone ID:2390

Experimenters: shanyi

Results

CLONING

PCR from Geobacillus stearothermophilus NUB3621 genomic DNA

2390-Gst-MutL-Nde1-F tagcacatatgggaaaaa ttcgcaagct c

2390-Gst-MutL-Xho1-R tgctactcgagcatcacccgcttaaacattttc

Digest with Nde1 + Xho1

Colony Screening

Date: 2014_12

Make miniprep, use Nde1 + Xho1to find positive clone

Result intepretation/Summary

Conclusion

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Cloning