2391 pEC2-pncA

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Cloning of2391 pEC2-pncA

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Purpose: Cloning of2391 pEC2-pncA==

Date: 2014-12----

Notebook:0

Page:

Clone ID:2391

Experimenters:  shanyi

Results

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CLONING

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gene synthesis


join two fragments by pcr



Digest with Nco1 + EcoR1


Colony Screening

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Date: 2014_12

Make miniprep, use  Nco1 + EcoR1 to find positive clone



fixing 2391_pEC2-pncA

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Purpose: Cloning of2391_pEC2-pncA==

Date: 2014-12_2015_1

Notebook:0

Page:

Clone ID:2391

Experimenters:  shanyi

Results

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CLONING

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use 2391-1 as template



join two fragment by pcr

clone to 2015 using Nde1 + Xho1


Colony Screening

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Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone




Result intepretation/Summary

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Conclusion

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