2326 pET30b-Ecoli-MutH

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Cloning of 2326 pET30b-Ecoli-MutH

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Purpose: Cloning of2326 pET30b-Ecoli-MutH==

Date: 2015--1-

Notebook:0

Page:

Clone ID:2326

Experimenters:  shanyi

Results

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CLONING

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PCR from Escherichia coli str. K-12 substr. MG1655 genomic DNA

2326-Ecoli-MutH-Nde1-F tacgacatatgtcccaacctcgcccactg

2326-Ecoli-MutH-Xho1-R tgctactcgagctggatcagaaaatgacgggcca



Digest with Nde1 + Xho1


Colony Screening

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Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone


Result intepretation/Summary

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Conclusion

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