2327 pL42-Tth-MutM

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Cloning of 2327 pL42-Tth-MutM

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Purpose: Cloning of 2327 pL42-Tth-MutM==

Date: 2015--1-

Notebook:0

Page:

Clone ID:2327

Experimenters:  shanyi

Results

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CLONING

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PCR from Tth HB27 genomic DNA

2327-Tth-MutM-Nde1-F ataatcatAtgcccgagcttcccgaggtg

2327-Tth-MutM-Xho1-R tgatactcgagggggccttccccctggcaggtg




Digest with Nde1 + Xho1


Colony Screening

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Date: 2015_1

Make miniprep, use Nde1 + Xho1 to find positive clone


Result intepretation/Summary

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Conclusion

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