2400 pET30b-hRAD51
Cloning of 2400 pET30b-hRAD51
[edit]Purpose: Cloning of2400 pET30b-hRAD51==
Date: 2015_1
Notebook:0
Page:
Clone ID:2400
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2397 pDNR-Dual-hRAD51
1
2400-hRAD51-Nde1-F
tagcaacatATGGCAATGCAGATGCAGCTTG
2400-hRAD51-R
GAACGCtcgCgcataGgcTACATTATC
2
2400-hRAD51-F
GATAATGTAgcCtatgcGcgaGCGTTC
2400-hRAD51-Xho1-R
(GAGTGGGAGATGCCAAAGACctcgag tagcaa)
ttgctactcgagGTCTTTGGCATCTCCCACTC
3
2400-hRAD51-Nde1-F
tagcaacatATGGCAATGCAGATGCAGCTTG
2400-hRAD51-Xho1-R
(GAGTGGGAGATGCCAAAGACctcgag tagcaa)
ttgctactcgagGTCTTTGGCATCTCCCACTC
digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + Xho1 to find positive clone
