2399 pET28b-scRAD52
Cloning of 2399 pET28b-scRAD52
[edit]Purpose: Cloning of2399 pET28b-scRAD52==
Date: 2015_1
Notebook:0
Page:
Clone ID:2399
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from 2395 ZM552-scRAD52
2399-scRAD52-Nco1-F tagatccatgggcagcagccatcatcatcatcatcacTCTCAAGTTCAAGAACAACATATATC
2399-scRAD52-Xho1-R (GACCCCAGAGAAGAAGACGAGTAG ctcgag tagcac) gtgctactcgagCTACTCGTCTTCTTCTCTGGGGTC
digest with Nco1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nco1 + Xho1 to find positive clone
