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Protein Expression

2015/1/23
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pEB3-Nco1	Nco1		15 ml, Tuner(DE3)plysN-hia5	0.2%Arabinose RT O/N	s	5.8/32.4	no
2						p		no
3				15 ml, Top10plysN-hia5		S		no
4						p		no
5	pageRuler Plus Prestained protein ladder
6	pMCSG7-hRAD52		2398	15 ml, Nico21(DE3)plysN	0.4mM IPTG RT O/N	s		yes
7	pL42-T4-uvsY		2408-3	15 ml, Nico21(DE3)plysN	42oc 4hours	s		no
8				15 ml, Tuner(DE3)plysN		s		no
9			2408-7	15 ml, Tuner(DE3)plysN		s		no
10				15 ml, Nico21(DE3)plysN		S		no
11	pEB3-uvsX		2392	1L Nico21(DE3)plysN	0.1%Arabinose 27oC O/N	S		yes
12				15 ml, Nico21(DE3)plysN	0.2%Arabinose 27oC O/N	s		yes