2198 pT7A3E-Tth-RecA
Cloning of 2198 pT7A3E-Tth-RecA
[edit]Purpose: Cloning of2198 pT7A3E-Tth-RecA==
Date: 2014-8----
Notebook:0
Page:
Clone ID:2198
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from HB8 genomic DNA using
Tth-RecA-Nde1-F
tagctacatatggacgagagcaagcgcaag
Tth-RecA-EcoR1-R
(tggccgcgggcgaggacgagggggag CACCACCACCACCACCACTGA gaattc tacgac)
gtgctagaattcTCAGTGGTGGTGGTGGTGGTGccccttcatgctacccagggggag
digest with Nde1 + EcoR1, clone to1982
Colony Screening
[edit]Date: 2014_8_
Make miniprep, use Nde1 + EcoR1 to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 2198 pT7A3E-Tth-RecA
[edit]Purpose: Cloning of2198 pT7A3E-Tth-RecA==
Date: 2015-1----
Notebook:0
Page:
Clone ID:2198
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from HB8 genomic DNA using
Tth-RecA-Nde1-F
tagctacatatggacgagagcaagcgcaag
Tth-RecA-EcoR1-R
(tggccgcgggcgaggacgagggggag CACCACCACCACCACCACTGA gaattc tacgac)
gtgctagaattcTCAGTGGTGGTGGTGGTGGTGccccttcatgctacccagggggag
digest with Nde1 + EcoR1, clone to1982
Colony Screening
[edit]Date: 2015_1_
Make miniprep, use Nde1 + EcoR1 to find positive clone
