736 pEC2-mNco1
Cloning of 736 pEC2-mNco1
[edit]Purpose: Cloning of736 pEC2-mNco1==
Date: 2014-9----
Notebook:0
Page:
Clone ID:736
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis
jion two fragments by PCR
DIGEST WITH NdeI+XhoI
Colony Screening
[edit]Date: 2014_8
Make miniprep, use NdeI + XhoI to find positive clone
fix 736-5 pEC2-mNco1
[edit]pcr
jOIN two fragment by PCR
digest with Nde1 + XhoI
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1 to find positive clone
Result intepretation/Summary
[edit]Conclusion
[edit]fix 736-5 pEC2-mNco1
[edit]pcr with Tth-muts
jOIN two fragment by PCR
digest with Nde1 + XhoI
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + PST1 to find positive clone
