1005-pBad24-Tat-sfGFP
Cloning of 1005-pBad24-Tat-sfGFP
[edit]Purpose: Cloning of 1005-pBad24-Tat-sfGFP by PCR
Date: 2012-2-10--2012-2-17
Notebook:003737
Page:
Clone ID:1005
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-10
Mix the His-Tat-NLS-1 to His-Tat-NLS-5 oligos and oligos for pBad24-sfGFP (546), gene synthesis
primer F:His-Tat-NLS-1
primer R:sfGFP20
| band 5 |
band 6 | |
| PCR product |
pBad24 545 | |
| digest enzymes |
Nco1 + HindIII | |
Colony Screening
[edit]Date: 2012-2-17
Make miniprep and digest with Nco1 + HindIII
clone 1,2,3,5,6 may be right, need to sequence
Result intepretation/Summary
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Conclusion
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