1012-pET28b-TurboRFP

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4/7/2012:

Gene synthesis using KAPA HIFi Ta, 50 ul reaction with 1 u Kapa HiF, 2 ul oligo mix

Looks like too much template is used

4/9/2012 PCR Screening

4/10/2012 mini-prep digest

1012-2 to sequence

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Cloning