Synthesis PHP-CBA-DSP-PEI18 SOP
Synthesis PHP-DSP-PEI SOP
[edit]PEI18: 4g/6 ml H2O (666 mg/ml)
1.5 g solid 1,3-Diamino-2-propanol, MW 90.12, 16.6 mmole
+3 ml MeOH
+3.63 g 1,3-dibrono-2-propanol , MW 217.89, 16.7 mmole
50 oC for 48 hours
store at -20 oc.
add 5 ml H2O to dissolve
+ 25 ml EtOH, mix, spin 3000 rpm for 5 min
remove supernatant
Add 5 ml H2O, + 25 ml EtOH
spin, repeat 2 more times
weigh wet pellet, calculate yield (usually 2.8 g wet PHP)
Add H2O to 10 ml to dissolve
Add 83 mg PEI18, & 83 mg DSP, reaction at RT o/n