282 pAAV-CMV-CFP
SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254)
[edit]Purpose: SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254).
Date: 2011-8-3 to 2011-8-10
Notebook:
Page:
Clone ID:
Experimenters: Robert, Mindy, Yolanda
Results
[edit]Subcloning
[edit]Date: 2011-8-4
Digest (clone #) with
Digest (clone #) with
cut out insert and vector, ligate and transform
Colony Screening
[edit]Date: 2011-8-10
Make miniprep and digest with BamH1 + Xho1
Lanes 1-4 + 6: pAAV CFP
Lane 5: 1 kb ladder
Result intepretation/Summary
[edit]- pAAV CFP clones
Conclusion
[edit]Restriction digestion
Shows