Yolanda Cloning

8/4/2011

1: 1 kb ladder marker

2: adx Lentivirus

• 1 uL adx Lentivirus
• 1 uL EcoRI (fast digestion)
• 1 uL BamHI (fast digestion)
• 1.5 uL 10x fast digestion buffer
• 10.5 uL water

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8/11/11

Digest with BamHI + XhoI

• 5 uL DNA
• 0.3 uL BamHI
• 0.3 uL XhoI
• 1.5 uL 10x buffer (fast digestion)
• 7.9 uL water

Total: 15 uL

Lane 1: 1 kb ladder

Lane 2: IFP 1, pET 28b

Lane 3: IFP 3, pET 28b

Lane 4: Amp pAAV-IFP 254-1

Lane 5: Amp pAAV-IFP 254-4

Notes:

• IFP 3 pET 28b still shows good insert
• Ran gel too long maybe

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8/12/11

Digest with BamHI + XhoI

Lanes 2-8 + 10-13:

• 5 uL DNA
• 1.5 uL 10x buffer (fast digestion)
• 0.5 uL BamHI
• 0.5 uL XhoI
• 7.5 uL water

Total: 15 uL

Lane 14:

• 10 uL DNA
• 3 uL 10x buffer (fast digestion)
• 1 uL BamHI
• 1 uL XhoI
• 15 uL water

Total: 30 uL

1: 1 kb ladder marker

2: IFP-3 pET28b

3: mNeptune-1 pET 286

4: mNeptune-2 pET 286

5: mNeptune-3 pET 286

6: sfGFP2 pET 286

7: Taq RFP-1

8: Taq RFP-2

9: 1 kb ladder marker

10: EGFP

11: Amp pAAV-IFP 2 (Mindy)

12: Amp pAAV-IFP 3 (Mindy)

13: Amp pAAV-IFP 5 (Mindy)

14: pAAV-CFP 4

Notes: