224 PLenti-cfp
SubCloning of CFP to pLentiplus
[edit]Purpose: Subcloning of cfp to pLentiplus from pAAV-CFP.
Date: 2011-8-15
Notebook: hAIYAN
Page: 003019
Clone ID: 224
Experimenters: Haiyan
Results
[edit]Subcloning
[edit]Date: 2011-8-15
Digest pAAV-CFP with BamH1 + Xho1
Run gel and cut out insert
Digest pLentiPlus with BamH1 + Xho1
Run gel and cut out vector combine and purify using spin column
Ligate and transform STBL3
Colony Screening
[edit]Date: 2011-8-15
Make miniprep and digest pLenti-CFP with BamHI + Xho1
Lanes 6-9: pLenti-CFP clone 1-4, BamHI + Xho1
Result intepretation/Summary
[edit]- clone 1,2,3 should be positive clone
Conclusion
[edit]finish clone 224
[edit]
User:Haiyan 11:33, 08/15/2011 (EST)