Real Time PCR Buffer for Blood

    Concentration  at 2.0X
Component    
1 M Tris-HCl pH 8.0    100 mM
5U/μl AmpliTaq Gold (or other Taq    0.5U/μl
25 mM each dNTP    0.4 mM
100 mM dUTP    0.8 mM
2 M KCl    100 mM
1 M MgCl2    8 mM
3 μM ROX Pass. Ref.    70 nM
20 mg/ml BSA (Roche)    0.08 mg/ml
10% NaN3    0.02%
50% Glycerol (or other polyhydroxy alcohol    16.00%
H2O    varying amount