Yidan 2016 9 27
Name: Yidan Hu
Date: Sept 27, 2016
EXP1
[edit]Purpose: Find out if home-made Nde1 enzyme is still good for plasmid DNA digestion
Procedure:
Digest 0.5ug plasmid 2411. Add 1ul of NdeI.
Result:
| 1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 | |
|
digestion enzyme |
HindIII |
NdeI 25ug/ml |
NdeI 50ug/ml |
NdeI 100ug/ml |
NdeI 200ug/ml |
NdeI 400ug/ml |
NdeI(old) 100ug/ml |
HindIII+ NdeI(25ug/ml) |
HindIII+ NdeI(50ug/ml) |
HindIII+ NdeI(100ug/ml) |
HindIII+ NdeI(200ug/ml) |
HindIII+ NdeI(400ug/ml) |
HindIII+ NdeI(old,100ug/ml) |
EXP 2
[edit]Purpose: 2477 pEC2-ALB, 2478 pEC2-betaActin,2482 pEC2-maize-actin1
Procedure: Make miniprep and digest with HindIII and NdeI.
Result:
2477 pEC2-ALB
2478 pEC2-betaActin
2482 pEC2-maize-actin1
