2489 pMiniT-TREC-ACTB-ALB
Cloning of 2489 pMiniT-TREC-ACTB-ALB
[edit]Purpose: Cloning of 2489 pMiniT-TREC-ACTB-ALB
Date: 2016-09-30
Notebook:
Page:
Clone ID: 2489
Experimenters: xxx
Results
[edit]PCR of 2489
Template: Trec-combo
Primer: 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R
PCR cycle:
1 min at 94 oC
30 cycles:
30 s at 94 oC
30 s at 55 oC
1.5 min at 72 oC
5 min at 72 oC
Make miniprep and digest with EcoRI.
PCR screening
Use Forward and reverse primer from Biolabs.
Send sample 1 and 5 for sequencing. Sample 5 is the desired product.
Result intepretation/Summary
[edit]Conclusion
[edit]Result intepretation/Summary
[edit]Conclusion
[edit][edit]