Purification of Taq DNA Polymerase
Buffers
[edit]Lysis Buffer
| 20 mM Tris-HCl, pH 8.0 |
|
| 500 mM NaCl |
|
| 0.5% NP 40 | |
| H2O | to 1 L |
Ni Washing Buffer 1
| 20 mM Tris-HCl, pH 8.0 | |
| 1 M NaCl | |
| 0.5% NP40 | |
| 10 mM Imadazole |
Ni Washing Buffer 2
| 20 mM Tris-HCl, pH 8.0 |
|
| 1 M NaCl | |
| 0.5% NP40 |
Ni Elution Buffer 1
| 20 mM Tris-Hcl, pH 8.0 | |
| 50 mM NaCl | |
| 150 mM Imadazole | |
| 5% Glycerol |
Ni Elution Buffer 2
| 20 mM Tris-HCl, pH 8.0 | |
| 50 mM NaCl | |
| 10 mM EDTA | |
| 5% Glycerol |
Q HP Buffer 1
| 20 mM Tris-HCl, pH 8.0 | |
| 50 mM KCl | |
| 5% Trehalose |
Q HP Buffer 2
| 20 mM Tris-HCl, pH 8.0 |
|
| 500 mM KCl |
|
| 5% Trehalose |