364 pET3a-MBP-TaqMuts

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Construction of pET3b-MBP-TaqMuts 364

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Purpose: construction of pET3b-MBP-TaqMuts (364).

Date: 2011-8-31 to 2011-9-

Notebook:

Page:

Clone ID:

Experimenters: Paul

Results

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Digestion

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Date: 2011-8-31

Digest  pET3a-Muts (clone #352) with Nde1+ Sph1

Digest  pDB.His.MBP (clone # 360 ) with Nde1+ Sph1

cut out insert MBP and vector pET3a-Muts , ligate and transform Top10

--Paul.xu 13:02, 31 August 2011 (EST)


Colony Screening

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Date: 2011-9-1

Result intepretation/Summary

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Conclusion

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Restriction digestion

Shows 

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