MS 4 5 2017
1458 KOD elute with Q HP column 04/05/2017
| Number |
A280 |
| 16 |
-0.02 |
| 17 |
-0.01 |
| 18 |
0.12 |
| 19 |
0.64 |
| 20 |
2.13 |
| 21 |
4.54 |
| 22 |
6.10 |
| 23 |
4.65 |
| 24 |
4.75 |
| 25 |
4.21 |
| 26 |
1.11 |
| 27 |
0.82 |
| 28 |
0.25 |
| 29 |
0.06 |
| 30 |
0.10 |
18~28
run SDS-PAGE gel
| number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| sample |
18 |
19 |
20 |
21 |
22 |
MW |
23 |
25 |
26 |
27 |
28 |
flow through |
| uL |
9 |
2 |
1 |
0.5 |
0.5 |
10 |
0.5 |
0.5 |
1 |
2 |
4 |
15 |
| ddH2O |
5 |
10 |
10 |
10 |
10 |
\ |
10 |
10 |
10 |
10 |
10 |
\ |
2013/3/29 lane3 http://excellgen.net/eln/index.php/1097_1138_1458_1494_1505_1513_1558_1562_1573_1593_1654
Combined 22 with 23, 24 with 25, concentrate these two samples with dialysis.
22/23: 7.44mg/ml 5.5ml, total 40.92mg. Wash#1 1.02mg/ml, 1ml. Wash#2 0.80mg/ml, 1ml.
24/25: 3.24mg/ml 6ml, total 19.44mg.
1458 KOD QC
| 22 |
23 |
positive control |
24 |
25 | |||||||||||||
| 1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
17 | |
| 1mg |
0.1mg |
10ug |
1ug |
1mg |
0.1mg |
10ug |
1ug |
1mg |
0.1mg |
10ug |
1ug |
1mg |
0.1mg |
10ug |
1ug | ||
| dNTP |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
| F |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
| R |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
| template |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
| 5x buffer |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
4 |
| H2O |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
12.4 |
| enzyme |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
Conclusion: 1ug/ml is good enough.