MS 5 23 2017
Plasmid transformation. 483/1464/1946/1497 transfer into Nico21(DE3) PlysN, culture with LB/K/C media.
Order shipment of 4865 (phi29 (φ29) DNA Polymerase).
Sorting refrigerator.
Order shipment of 4866 (HTN-GFP, Cell-Permeable Green Fluorescent Protein).
Culture 1613 1782 2196 1498 2295 for expression screening.
run SDS-PAGE gel 1811VPol / 1995Kfu/ 2409iFLP/ 2295yPolyA-Pol/ 1458KOD / 1715Pfu / 1315Mth RNA Ligase 05/23/2017
| Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
| sample |
1811 |
1995 |
2409 1 |
2409 2 |
2295 1 |
2295 2 |
1458 1 |
1458 2 |
MW |
1715 13 |
1715 16 |
1315 41 |
| uL |
0.2 |
0.4 |
1.8 |
1.8 |
1.5 |
1.2 |
0.2 |
0.4 |
10 |
0.8 |
1.3 |
0.5 |
| ddH2O |
15 |
15 |
15 |
15 |
15 |
15 |
15 |
15 |
\ |
15 |
15 |
15 |
