MS 5 24 2017

Use IPTG to induce 1613pET28-cold-GST-3C 1782Tth-Ligase 2196PBCV-1-Lig 1498TaqDNALigase 2295yPolyA-Pol for expression screening. Spin down cells, wash with Lysis Buffer, break down through blunted needle, and incubate with 50 ul regenerated Ni beads. Wash Ni beads, prepare to run SDS-PAGE gel.

Select one colony to culture 483 Tth Polymerase /1464 T7 DNA Ligase / 1946 TdT bovine / 1497 ToughTaq in 15ml LB/K/C media.

Quote for Dr. Kato, GRred.

Deal with order 4867.

Data management.

Cell culture, 1708 HTNCre.