MS 6 1 2017

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Culture 1708 HTN Cre (8L), induced with IPTG, over night.

Spin down 483 Tth Polymerase.

Dialysis of 1708 HTN Cre, total 37ml, 1.07mg/ml. Need to further dialyse.

Culture 1464 T7 DNA ligase (200ml)

Wash Ni column (bind with 1782 Tth ligase) with Ni washing Buffer/ Tris-HCl/ Buffer B,

Elute 1782 Tth ligase with Buffer B 06/01/2017

-0.08
 0.12
 1.07
 3.23
 5.68
 (1~5)
3.70
 2.49
 1.46
 0.87
 0.59
 (6~10)
0.43
 0.31
 0.30
 0.19
 0.11
 (11~15)

load sample 3~12 onto QHP column.

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