MS 6 5 2017
Wash Ni column (load with 483 Tth Polymerase) with Ni washing buffer / Tris-HCl / Buffer B, dilute and load onto QHP column.
Elute 483 Tth Polymerase with Buffer B 06/05/2017
| -0.03 |
-0.02 |
-0.08 |
-0.12 |
-0.13 |
(1~5) |
| -0.11 |
-0.05 |
0.13 |
0.50 |
1.18 |
(6~10) |
| 2.92 |
1.33 |
0.51 |
0.27 |
0.17 |
(11~15) |
| 0.17 |
0.08 |
0.04 |
0.04 |
0.03 |
(16~20) |
Culture 1464 T7 DNA Ligase 8L, induce with IPTG.
Culture 1946 TdT bovine 200ml.
Prepare LB/Amp agar.
Plasmid transformation of ligation sample 1 and 2.
Mini prep of 1420
Prepare LB media.
Deal with order 4873.