Cre Activity Assay
Grow 293T in 12 well plate.
Transformation of 293T cells with 1 ug 2181 pCMV-LoxP-kozak-TurboRFP-ODC-2Stops-LoxP-TurboGFP.
Change medium
Add Tat-Cre at 1, 2.5, 5 uM
Grow 293T in 12 well plate.
Transformation of 293T cells with 1 ug 2181 pCMV-LoxP-kozak-TurboRFP-ODC-2Stops-LoxP-TurboGFP.
Change medium
Add Tat-Cre at 1, 2.5, 5 uM