MS 6 6 2017
Spin down 1464 T7 DNA Ligase, homogenize, centrifugate and load onto Ni column.
Culture 1946 TdT bovine 8L, induce with IPTG.
Culture 1497 sso7d-ToughTaq 200ml.
Elute QHP column (load with 483 Tth Polymerase)
Run SDS-PAGE gel of 1782 Tth ligase and 483 Tth polymerase 06/06
|
1782 Tth ligase 79.1 |
483 Tth polymerase 96.1 | |||||||||||
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
11 |
12 |
16 |
MW |
10 |
12 |
14 |
15 |
16 |
18 |
19 |
20 |
|
uL |
0.6 |
0.2 |
2.7 |
10 |
2.4 |
1.6 |
2.0 |
2.2 |
1.6 |
2.1 |
3.2 |
6.7 |
|
ddH2O |
15 |
15 |
10 |
\ |
10 |
15 |
10 |
10 |
15 |
10 |
10 |
10 |
Dialyse sample 11 and 12 of 1782 Tth ligase, 19 and 20 of 483 Tth polymerase.
1782 Tth ligase totally have 10ml, 10.64mg/ml, 106.4mg. 483 Tth polymerase totally have 13ml, 1.11mg/ml. 14.43mg. Store at -80oC.