MS 6 8 2017

Culture 1483 T4 DNA Ligase 200ml.

Culture 1498 Taq DNA Ligase 8L, induce with IPTG.

Spin down 1497 sso7d-ToughTaq.

1946 TdT bovine homogenize, centrifugate and load onto Ni column.

1942 phi 29, 1601 6His-GST-i3C, 1518 T3 Ligase, 1765 T4 DNA Polymerase pick up single colony each to culture in LB/K media for glycerol stock.

1942 phi 29, 1601 6His-GST-i3C, 1518 T3 Ligase, 1765 T4 DNA Polymerase, transfer into Nico21, culture on LB/K/C agar over night.

Elute QHP column (load with 1464 T7 DNA Ligase)

Elute SP FF column (load with 1708 Cre Recombinase) , combine fration with OD₆₀₀>2.5, dialysis through dialysis bag against Cre dialysis buffer.

Prepare Storage buffer, SP Wash Buffer, SP Elution Buffer.

Lable transfection reagent.