Toxic Minigene

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5'-C UGAACGAGAAACGU AAA AUGAUAUAAAUAUCAA. 3'  barl

$ ' -CAUCAGGA AUAUUUGAUUCAG AUG AUA  UGA CUAUCAA- 3'  bar ll

Met Ile stop

barI and barII siles and their nucleotidc sequences in the lambda genome. The double line (top) represents Ihe complete lambda. genome. The
first enlargent (middle) shows the genetic context surrounding bari and barII loci The second enlargement (bottom) shows the homologous
sequence in barI and BarII, Nucleotides forming part of the open reading frames of  barl and barll are indicated by arrows, b. Sequences of barI
barII transcrirrs, Underlined nucleotides are the S-D seqnences and those in boldface indicate the putative ORFs of bar minigenes.

pMiniT

SP6 promoter

CAT TTAGGTGACACTATAGA   AGTGTGTATCGCTCGAGGGATCCGAATTCAGGAGGTAAAAACC ATG ATC TGA TAA TAA TTA

Minigene: ATG ATC TGA (Met Ile Stop)

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