MS 6 15 2017
Troubleshoot the low flow speed problem of Ni column. Wash Ni column (bind with 1483 T4 DNA Ligase) with Ni washing buffer.
Elute QHP column (load with 1498 Taq DNA Ligase)
Run SDS-PAGE gel of 1497 ToughTaq 7.40/72.2 06/15/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
22 |
23 |
25 |
MW |
26 |
27 |
28 |
29 |
30 |
31 |
33 |
35 |
|
uL |
3.5 |
0.7 |
2.7 |
10 |
2.2 |
3.0 |
2.2 |
2.4 |
2.2 |
1.6 |
2.2 |
4.6 |
|
ddH2O |
10 |
15 |
10 |
\ |
10 |
10 |
10 |
10 |
10 |
15 |
10 |
10 |
Forward sample 22~24 to the next step.
Prepare Buffer A.
Run SDS-PAGE gel of 1498 Taq DNA Ligase 6.56 / 79.1 06/15/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
sample |
20 |
22 |
23 |
24 |
26 |
27 |
MW |
28 |
29 |
32 |
35 |
39 |
|
uL |
4.8 |
0.8 |
0.3 |
0.2 |
0.6 |
0.9 |
10 |
0.8 |
0.7 |
1.4 |
2.7 |
4.2 |
|
ddH2O |
10 |
15 |
15 |
15 |
15 |
15 |
\ |
15 |
15 |
15 |
10 |
10 |
Forward sample 37~39 to the next step.
Dialysis. 1946 TdT bovine, 3.3mg/ml, 2.0ml, 6.6mg. Store at -80oC, 1-1-2 (Enzyme 2017)