MS 6 21 2017
Culture 1697 pCold-GST-(3c)-wt3C 200ml.
Culture 1613 pET28-cold-GST-3C 8L, induced with IPTG at 16oC.
Homogenize 1942 phi29, load onto Ni column.
Elute QHP column (load with 1483 T4 DNA Ligase)
Run SDS-PAGE gel of 1483 T4 DNA Ligase 6.63 / 57.3 06/21/2017
|
Number |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
|
sample |
10 |
11 |
12 |
13 |
14 |
15 |
16 |
MW |
|
uL |
8.4 |
0.16 |
0.13 |
0.6 |
1.2 |
2.5 |
7.2 |
10 |
|
ddH2O |
5 |
15 |
15 |
15 |
15 |
10 |
5 |
\ |
forward lane 1~3 to the next step.
Dilute 2295 yPolyA and load onto QHP column.
Elute QHP column (load with 2295 yPolyA)
Miniprep. 2462 Tn5 transposase.
Plasmid transformation of 818 N-6His, Internal 6His. Flag, C-6His / 819 N-His, GST, Myc, C-His / 820 N-6His, GST, HA, C-6XHis / 1708 HTNCre / 2462 Tn5 transposase